Research and Development Services

Experts in biochemistry, biophysics, and molecular biology with a variety of research related services including, but not limited to:

Design, Express, and Purify Recombinant Proteins

All proteins are expressed and purified with the utmost attention to every detail. Options are available for high purity, low endotoxin levels, conjugations, lyophilization, and formulation studies. All expressed and purified proteins are validated using SDS PAGE, Western blot, and gel filtration (if requested).

  • Plasmid customization and conceptualization
  • Prokaryotic and Eukaryotic expression systems
  • Very high degree of success with difficult to express and to purify proteins
  • Affinity tagged and native purifications
  • Milligram to grams scale
  • Protocol development for transfer or protein made to order, tailored to your needs
  • Experts in the relationship between structure and function of proteins

Protein Modifications

  • Conjugation chemistries
  • Add and/or remove functions or characteristics of proteins
  • Conjugations of PEG, targeting peptides, cytotoxins, etc.

Protein Therapeutics

  • Enzymes
  • Protein conjugates
  • Humanized monoclonal antibodies
  • Fusion proteins
  • Cytokines
  • Receptor decoys

Assay design, development, and implementation

  • Completely customizable depending on your research goals and needs
  • Cell and protein based assays
  • Enzymatic assays
  • Mechanistic determination of allosteric, competitive, and uncompetitive inhibition and activation
  • Protein-Protein interaction assays

Drug Discovery efforts, including:

  • High throughput screening
  • Small, large, and fragment based screens
  • Discovery, development, and validation of your target interactions
  • 96, 384, (and 1536) well plate formats available

Target Validation

  • Stable or transient siRNA Knockdowns using your target gene and cell line combination
  • Full range of reporter proteins available, such as GFP and RFP, in addition to customizable selection markers
  • Inducible shRNA Expression systems available
  • Stable knockdowns can be provided as polyclonal or monoclonal cell lines

In Vivo Services

The Veterinary Resources at PMI BioPharma are committed to providing high quality research models. Our main objective is to satisfy the needs of our clients in a timely and effective manner while ensuring humane care is given at all times. Every associate in this department is exceptionally trained and capable to adapting to any situation given by a client. This department currently has access to rat and mouse models with the capability of future expansion to other models.

Monoclonal Antibody Development

PMI BioPharma has a highly dedicated and competent team of scientists solely dedicated to the production of high quality monoclonal antibodies. Our expertise in tissue culture, biochemistry, molecular biology, chemistry, biology and bioengineering ensures that our final product meets the highest standards.  PMI BioPharma can also rapidly create recombinant and humanized versions of monoclonal antibodies for therapeutic applications.

Additional Research Services

  • Protein engineering
  • Cell line development
  • Pre-formulation stability studies
  • Pre-GMP upstream and downstream development
  • Full develop-ability and manufacture-ability assessment

State of the art equipment and concepts designed for efficient and timely turnaround

  • Shaker flask or stirred (up to 20L) tank bioreactor expression systems available
  • Microfluidizer or sonication lysis options available.
  • Expression systems also in place for excreted protein purifications in both prokaryotic and eukaryotic systems.
  • GE Avant Fast Protein Liquid Chromatography (FPLC)
  • Affinity tag purifications including IMAC, Protein A (endotoxin free), GST, FLAG, etc.
  • Ion exchange, hydrophobic interaction, gel filtration, and multimodal chromatography
  • Charles River Endosafe Endotoxin testing system for accurate endotoxin levels
  • Formulation studies performed with Differential Scanning Fluorometry
  • Tecan Freedom Evo liquid handler
  • Tecan Spark 10M microplate reader
  • GE Biacore S200 (the most sensitive surface plasmon resonance technology available on the market).
  • Small molecule colloidal propensity, aka surface tension analysis
  • Differential scanning fluorometry
  • Isothermal Calorimetry
  • Dynamic Light Scattering